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M9 minimal salt medium containing 5 g/L glucose, 15.1 g/L Na 2 HPO 4 , 3 g/L KH 2 PO 4 , 0.5 g/L NaCl, 1 g/L NH 4 Cl, 0.5 g/L MgSO 4 7H 2 O, 0.01 g/L CaCl 2 , 0.2 mL/L 1 % vitamin B1 and a 0.05 mL/L stock solution of trace elements was used for the cultivation of engineered E

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Results Generation and screening of FGF5 KO sheep Since we have investigated the efficiency of detecting mutations by PCR sequencing (Supplementary Table S1), the blood DNA template was screened for positive individuals using PCR and DNA sequencing (Supplementary Fig